Antimicrobial effectiveness of photodynamic therapy against endodontic pathogens
Keywords:
Photodynamic therapy, Methylene blue, EndodonticsAbstract
Endodontics aims to sanitize the root canal through instrumentation and the use of
chemical substances. Even though the success rate is high, there are cases where the
infection of the canal system persists. Thus, the present study aimed to evaluate the
antimicrobial efficacy of methylene blue in different concentrations associated with
photodynamic therapy against endodontic pathogens. The study is characterized by an
experimental in vitro study and was conducted using standard plate count methodology.
The analyzed samples were divided into two subgroups, the first in 0.005% methylene
blue (MB 0.005%) and the second in 0.01% methylene blue (MB 0.01%). Then, the
antimicrobial action against the main endodontic pathogens was evaluated: S. aureus, C.
albicans, P. aeruginosa, E. coli and E. faecalis. Among these concentrations, the best
results were obtained at the 0.005% concentration, which, compared to the 0.01%
concentration, showed greater effectiveness. Given the conditions of the present study, it
is possible to say that the results were satisfactory, mainly for the 0.005% concentration,
which is usually used in Endodontics. The association of methylene blue to PDT can be
considered a local and non-invasive adjuvant therapy, favorable to endodontic treatment
in the face of persistent infections, acts in the reduction of antimicrobial resistance.
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